Genomic and genetic industries are considered among the fastest growing biotech markets in the world, yet the underlying DNA analysis results are often inaccurate or misleading due to samples with damaged DNA and artifacts from storage.
Difficult FFPE Samples
While FFPE samples can be stored stably at room temperature for years, the fixation process creates inter- and intra-strand cross-links in the DNA. These cross-links prevent amplification of the sample and can render certain parts of the genome inaccessible or even entire failure of the sequencing run.
poor RNA-Seq Results
Transcriptomics applications, like RNA-Seq and RNA microarrays, require a series of enzymatic steps resulting in the creation of cDNA libraries. Libraries contaminated with DNA/RNA hybrids and ssDNA will throw off conventional QC methods, leading to an overestimation of library material.
Library Cloning Failure
The enzymes involved in cloning DNA molecules require dsDNA. Samples contaminated with ssDNA can result in either lower yields or even complete failure of the library.